Biosynthesis, characterization, and antibiofilm activity of copper-oxide nanoparticles using a mixture of Klebsiella pneumonia and Staphylococcus aureus supernatant

Biosynthesis of copper oxide nanoparticles using the extracellular mixture solution of Gram-negative bacteria Klebsiella pneumonia and Gram-positive bacteria Staphylococcus aureus acted as a reducing and capping agent to stabilize and prevent the aggregation of the biosynthesized nanoparticles. CuO NPs are characterized by UV-visible, FTIR, AFM, FE-SEM, and XRD, and tested for their antibiofilm activity against Pseudomonas aeruginosa. The biofilm capability was demonstrated with the microtiter plate method. The antibiotic susceptibility of P. aeruginosa exhibits resistance to Piperacillin, Nitrofloxacin, and Levofloxacin. While sensitive to Cefepime, Piperacillin-Tazobactam, and Imipenem.   The antibiofilm activity of CuONPs illustrates that all the prepared concentrations exhibit antibiofilm efficacy against P. aeruginosa biofilm, and 50 mg/ml was correlated with the highest activity. The concentrations 25, 12.5, and 6.25 mg/ml reduced the biofilm intensity to a weak level, and the last effect was associated with a 6.25 mg/ml concentration.