Like any other information system, the Accounting Information System (AIS) is perceived as an integral part of management at different stages within the organization. It is composed of different interdependent subsystems that generally work together to compile the AIS as a framework to provide ideal, reliable, and precise information, which facilitates the managerial decision-making process within organizations promptly, based on which the survival and effectiveness of an organization depend. The researchers couldn't recognize notable studies in recent times that assessed the role played by AIS on the profitability of Bangladeshi private commercial banks. Being deeply concerned about this fact, the present study attempts to investigate the impact of AIS on the profitability of the selected private commercial banks in Bangladesh. A self-structured questionnaire, set on a five-point Likert scale (consisting of both dependent & independent variables), has been used to rate the collected primary data. From 265 respondents, data were collected, and linear regression was applied to analyze the data using SPSS software. The results from the analysis have revealed that there is a significant impact of AIS on the selected banks’ profitability, except Jamuna Bank Ltd., which discloses the relationship between the AIS used by organizations and their profitability. Finally, it recommends that a successful implementation of AIS is expected to be beneficial for the banking sector of Bangladesh to stimulate profitability. Again, in the future, the paper is to be considered helpful for conducting new research in a wider scope.