A glucometer-colorimetric dual mode biosensor for point-of-care testing of cardiac troponin I

cTnI is considered the primary marker for the diagnosis of Acute myocardial infarction (AMI).. At present, cTnI relies on laboratory testing, which requires technical personnel and specialized equipment. This strategy proposes a dual-mode sensor based on a blood glucose meter and colorimetry to achieve portable, rapid, and accurate measurement of cTnI. The feasibility of this sensor was validated using UV spectrophotometry and polyacrylamide gel electrophoresis. Under optimal conditions, the linear ranges for the glucometer and colorimetric modes were 0.01 ngmL-1 to 20.0 ngmL-1 and 0.03 ngmL-1 to 15.0 ngmL-1, with detection limits of 0.001 ngmL-1 and 0.005 ngmL-1, respectively. The dual-mode sensor exhibits high sensitivity and selectivity, enabling rapid portable dual-mode detection. This method has been successfully applied for detecting cTnI in human serum, indicating significant potential for clinical applications.