Molecular detection of some virulence factors of hypervirulent Klebsiella pneumonia that associated with pathogenicity

Abstract: The study amid to isolation and identification of Hypervirulent K. pneumonia (hvKp) from  clinical samples and virulence factors genes was  detected. The present study included specimens collected from (Urine, Diabetic Foot, sputum , Liver abbesses , Blood , CSF ,and  Semen ,) of patients infected with K. pneumonia  in hospitals in Najaf City for both sexes,  Male were  (34) and Female (47) of age ranged between (10-50) years during the  period September to December - 2022.
    Eighty (80/100) isolates of K. pneumonia were detected by Morphological, microscopy and biochemical and confirmed by VITEK-2 system  as a (hvKp).    The result of phenotypic detection of some virulence factors such as  biofilm , capsule, sidrophore, Outer membrane Protein(omp) and haemolysis), all isolates were biofilm producers(100%), with three  different categories. According to the results of screening virulence genes, BssS (n= 80, 100%) was prevalent among all the clinical isolates, followed by the rmpA (n=22, 27.7%), and magA (n=3 .7%),