Diabetes Mellitus (DM) is One of recognized metabolic disorder that can be distinguish by its chronic character, abnormalities in metabolism of proteins, lipids, carbohydrates, and hyperglycemia. Due to its extensive prevalence roughly one in ten people worldwide it's crucial to recognize and treat this illness. The revised goal is Investigating the complex interactions between insulin and insulin resistance is goal of this study.; (IR), lipid profiles, and C-peptide and their levels in the Basrah, Iraq, population with Diabetes Type 2 (T2DM). Furthermore, we aim to discuss the clinical significance of these findings and their implications for T2DM management in this region. In our investigation, significant differences (p < 0.001) were observed in parameters like Insulin and C-Peptide between T2DM patients and the control group. Other parameters like HbA1C%, FBG, Cholesterol, Triglyceride, HDL, and LDL showed varying significance levels, with p-values ranging from 0.001 to 0.002. Our study highlighted substantial distinctions between individuals with T2DM and healthy control group across multiple critical parameters. These differences, I mean elevated C-peptide, insulin, HbA1C, FBG, cholesterol, triglyceride, and LDL levels in T2DM patients suggest the potential influence of these markers on severity of condition