Research aim is to identify mediating role of strategic knowledge in strengthening relationship between strategic leadership and achieving strategic ambidexterity in our Iraqi organizations. The intellectual research problem was represented by main question: Does strategic leadership have an impact on achieving strategic ambidexterity through mediating role of strategic knowledge? On basis of which sub-questions were raised through which appropriate solutions could be found for research community company. Importance of research was evident from importance of field of application, which has become a need to provide various solutions to advance and develop productive sector. Research was applied in General Company for Grain Processing - Holy Karbala on an intentional sample of (58) respondents from its administrative, technical and engineering staff, based on a questionnaire prepared according to lekart scale. A set of statistical methods were used to analyze data, including simple correlation coefficient (Pearson) and discriminant validity test and Cronbach's alpha for validity and reliability of search scale by using statistical program SPSS V.25 & Smart PLS V.20. As research reached a set of results, most important of which is existence of a direct correlation and positive effect with a moral significance between research variables, as well as an indirect effect with a moral significance for strategic knowledge in relationship between strategic leadership and achievement of strategic ambidexterity in company.