The roots of Chrysopogon zizanioides (L.) Roberty, belonging to the Poaceae family, are the main source of volatile that has substantial value in perfume, cosmetics, pharmaceutical and food industries. The study of C. zizanioides volatiles, obtained from plant roots cultivated in Tunisia, using GC and GC/MS revealed that more than 50 compounds were detected of which 14 constituents were identified representing 33.87% of the total. Oleic acid (17.09%) and (E) -3-Eicosene (7.77%) were the major components at the species level. However, 10,13 methyl ester octadecadienoic acid (0.01%), (Z) - 9,17-octadecadienal (0.01%), (Dimethylethyl-1,1) Bis-2,6 Methyl- 4 Phenol (0.04%), methyl pentadecanoic acid, 13-methyl-, methyl ester (0.05%), (Z) -13-octadecenal (0.06%) and linoleic acid ethyl ester (0 , 06%) are the constituents which have the lowest contents.